Plasmid_Backbone

Part:BBa_K4415000:Design

Designed by: William Pihlainen-Bleecker   Group: iGEM22_McMaster_A   (2022-09-30)


f3-55nm-MCS chimeric phage


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal XbaI site found at 1431
    Illegal PstI site found at 8502
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal PstI site found at 8502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal BglII site found at 8498
    Illegal BamHI site found at 5065
    Illegal XhoI site found at 8494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal XbaI site found at 1431
    Illegal PstI site found at 8502
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 745
    Illegal XbaI site found at 1431
    Illegal PstI site found at 8502
    Illegal AgeI site found at 309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

To include both the MCS and the protein display insertion sequence, f3-55nm and fMCS were combined. Both were digested with BamHI-HF and SacII; with the larger fragment of f3-55nm used, and the shorter fMCS fragment used. They were then mixed and ligated with T4 ligase.

Source

Created using the f3-55nm and fMCS phages supplied to us by Dr. George P. Smith

References